Classes for Spring 2012 Classes/Sum 2012 Courses Offered Useful Scientific links List of all the courses taught: BIO L 4531 Molecular Biology BIOL 4522 Molecular Biology Laboratory BIOL 4523 Lab for Biotech BIOL 4534/ CSCI 4931 Intro to Bioinformatics BIOL 4436 Pathophysiology BIOL 4728 Seminar in Biology BIOL 4631 Immunology BIOL 5132 Advances in Molecular Biology BIOL 5731/ BIOT 5031 Applied Biotechnology BIOL 5931 Research Topics in Biology: Patho-physiology BIOT 5131 Advanced Methods in Biotechnology I BIOT 5132 Advanced Methods in Biotechnology II BIOL 5738 Gene Therapy BIOL 5530 Research Methods BIOL 6838 Research Project & Seminar	M. Bazlur Rashid Associate Professor Biology & Biotechnology Natural Sciences Division School of Science and Computer Engineering Tel: (281) 283-3756 Fax:(281)-283-3709 E-mail: rashid@uhcl.edu Address: 2700 Bay Area Blvd., Houston, TX 77058 Education Ph.D. (Molecular Genetics) 1994 Osaka University Medical School, Japan. MD (Medicine) 1986 Dhaka Medical College, University of Dhaka, Bangladesh AWARDS/Scholarships Ruth L. Kirschstein National Research Service Award fellow, 2000 Monbusho Scholarship (Ministry of Education, Science & Culture, Japan), 1989 Bangladesh National Board Scholarship for Medical Education, 1980 Training/Employment National Research Service Award Fellow     03/00- 01/03 -Dept. of Medicine, Baylor College of Medicine, Houston, TX Post-doctoral Research Associate   09/94- 02/00 -Cellular Biology, University of Georgia Medical Officer/ Assistant Surgeon  06/87-06/89 -Ministry of Health, Bangladesh Intern  04/86-05/87 -Dhaka Medical College Hospital, Dhaka, Bangladesh general Research Interests Regulation and localization of proteins/enzymes aiming towards vaccine development or chemotherapy Cloning of novel genes to study roles in pathophysiology in human diseases Universal expression of genes in both prokaryotes and eukaryotes Mechanism of DNA replication GRANTS · UHCL Faculty Research Support Fund(2003-2010): $ 22,790 · NIH-NICHD AREA Grant: New Human Inducible Nitric Oxide Synthase-binding Proteins in Testis: Possible Role in Fertility. $341,934 (Funded; funding ends September 2013) -PI RESEARCH ACTIVITIES Nitric oxide (NO) is a cell signaling molecule and involved in numerous biological processes, including vasodilatation, neurotransmission, macrophage mediated immunity and carcinogenesis. Nitric oxide (NO) is differentially formed in almost all types of human cells by two constitutive and one inducible form of nitric oxide synthases (NOS). NO synthesized by eNOS and nNOS are beneficial for the physiological processes in cells. Inducible form of NOS, however, synthesizes NO in excess, which may prove to be harmful to cells. This may also be the reason for the inflammation based infertility in males as a result of reduced motility of sperm cells in testis. Hence it has become necessary to specifically inhibit iNOS to cure inflammation based infertility as well as number of other conditions involved with increased NO. One way to inhibit iNOS is by targeting proteins that interact with it. Therefore, identification of those iNOS-interacting key proteins are essential which should not affect other NOSs thus maintaining the physiological NO level. We can achieve this by screening the human testis cDNA library using iNOS as a bait employing a yeast two-hybrid system. PUBLICATIONS Kolodziejski PJ, Rashid MB and Eissa NT, 2003. Intracellular formation of "undisruptable" dimers of inducible nitric oxide synthase. Proc Natl Acad Sci U S A. Nov 25;100(24):14263-8 Rashid MB, Stanton JD and Mensa-Wilmot K, 2002. Cysteine-less GPI-phospholipase C is Inhibited Competitively by a Sulfhydryl Reagent: Glyco-mimicry by para-Chloromercuriphenylsulfonate (Biochem J. Aug 15; 366(Pt 1): 281-8) Ghosh DK, Rashid MB, Crane B, Taskar V, Mast M, Misukonis MA, Weinberg JB, Eissa NT, 2001. Characterization of key residues in the subdomain encoded by exons 8 and 9 of human inducible nitric oxide synthase: a critical role for Asp-280 in substrate binding and subunit interactions. Proc Natl Acad Sci U S A. Aug 28; 98(18): 10392-7 Rashid MB and Mensa-Wilmot K, 2000. A novel kanamycin/neomycin phsophotransferase cassette increases transformation efficiency of E. coli. Biotechniques (January) Rashid MB, Russell M and Mensa-Wilmot K, 1999. Roles of Gln81 and Cys80 in Catalysis by GPI-Phospholipase C from Trypanosoma brucei. Eur J Biochem. Sep 15; 264(3): 914-920. Teilhet M, Rashid MB, Hawk A, Al-Qahtani A and Mensa-Wilmot K, 1998. Effect of short 5’ UTR on protein synthesis in two biological kingdoms. Gene 222: 91-97. Rashid MB, Shirahige K, Ogasawara N and Yoshikawa H, 1994. Anatomy of the stimulative sequences flanking the ARS consensus sequence of chromosome VI in S. cerevisiae. Gene 150: 213-220. Yamazoe M, Shirahige K, Rashid MB, Kaneko Y. Nakayama T, Ogasawara N, Yoshikawa H, 1994 A protein which binds preferentially to single-stranded core sequence of autonomously replicating sequence is essential for respiratory function in mitochondria of S. cerevisiae. J Biol Chem. 269: 15244-15252. Shirahige K, Iwasaki T, Rashid MB, Ogasawara N and Yoshikawa H,1993. Location and characterization of autonomously replicating sequences from chromosome VI of Saccharomyces cerevisiae. Mol Cell Biol 13: 5043-5056. Presentations/Abstracts American Thoracic Society, San Francisco, CA. May'01; Characterization of Key residues in the region of exons 8-9 of human iNOS: Asp280 is critical for substrate binding and subunit interactions FASEB Experimental Biology, Orlando, FL. Mar'01; Characterization of Key residues in the region of exons 8-9 of human iNOS. Molecular Parasitology Meeting X, Marine Biological Laboratory, Woods Hole, MA Sep'99. Contribution of non-covalent interactions to inhibition of GPI-Phospholipase(s) C by a sulfhydryl reagent: identification of a putative target for p-CMPS inhibition of GPI-PLC from T. brucei. Molecular Parasitology Meeting IX, Marine Biological Laboratory, Woods Hole, MA. Sep'98; Possible roles of Gln81 and Cys80 in Catalysis by GPI-Phospholipase C from T. brucei. Cellular Biology Departmental Seminar, University of  Georgia, Athens, GA. Jan'96; Anatomy of the autonomously replicating sequences in S. cerevisiae. Annual Molecular Biology Meeting, Tokyo, Japan, Dec'93; Study of the stimulative sequences of ARSs on chromosome VI of S. cerevisiae. Annual Molecular Biology Meeting, Kyoto, Japan, Dec'92; Title: Fine analysis of the type I ARSs (Autonomously replicating sequences) on chromosome VI of S. cerevisiae	UNDERGRADUATE RESEARCH OPPORTUNITIES: UNDERGRADUATE STUDENTS ARE ENCOURAGED TO JOIN MY RESEARCH TEAM ON AN NIH PROJECT. CONTACT IMMEDIATELY IF YOU ARE INTERESTED.